Chromatographic Performance of an Amine/amino-bonded Column and a Monolithic Reversed-Phase Column for the Separation of Fluorescently Labeled Glycoprotein Glycans
نویسندگان
چکیده
منابع مشابه
Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column
The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...
متن کاملSeparation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column
The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...
متن کاملDevelopment of Monolithic Column Materials for the Separation and Analysis of Glycans
Monolithic column materials offer great advantages as chromatographic media in bioseparations and as solid-supports in biocatalysis. These single-piece porous materials have an interconnected ligament structure that limits the void volume inside the column, thus increasing the efficiency without sacrificing the permeability. The preparation of monolithic materials is easy, reproducible and has ...
متن کاملDesign and Fabrication of an Improved Single-Column Chromatographic Separation Process
In this work, an improved single-column chromatographic (ISCC) separation process is proposed. The term `improved' refers to both conceptual and physical modifications compared to the available single-column processes, including a novel fraction collection scheme and allowing overlapped peaks from adjacent cycles. Also the fraction collection mechanism was modified in order to facilitate online...
متن کاملseparation of somatropin and its degradation products by high-performance liquid chromatography using a reversed-phase polymeric column
the accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. in this study, a gradient rp-hplc method is described for the separation of human growth hormone (hgh) variants as deamidated and oxidized forms. the methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1ml/min flow rate of a linear gradient of 0.1% v/...
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ژورنال
عنوان ژورنال: CHROMATOGRAPHY
سال: 2021
ISSN: 1342-8284,1348-3315
DOI: 10.15583/jpchrom.2021.005